ytzG [2019-03-19 19:30:20]

similar to 16S pseudouridylate synthase

locus: BSU30035

pI: 5.62

mw: 27.00 kDa

protein length: 239 aa

gene length: 720 bp

function: 16S rRNA modification

product: putative 16S pseudouridylate synthase

essential: no

ec: 5.4.99.19

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.3|rRNA modification and maturation/ based on similarity]

Gene

Coordinates: 3,072,743 3,073,462

The protein

Catalyzed reaction/ biological activity

• uridine⁵¹⁶ in 16S rRNA a pseudouridine⁵¹⁶ in 16S rRNA (according to E. coli RsuA)

Protein family

• [SW|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• pseudouridine synthase rsuA family (according to Swiss-Prot)

[SW|Domains]

• [SW|S4 RNA-binding domain] (aa 1 ... 66) (according to UniProt)

Structure

• [PDB|1KSK] (RsuA from E. coli, 40% identical) [pubmed|11953756]

Expression and Regulation

Operons

• genes: [gene|0AFFB4B715CCE6B1C53BBB7F890EFB3126CB466E|murJ]-[gene|69052B24B577ABA4B20E610F679154DC97EFD8DE|ytzG]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE30035 ([gene|69052B24B577ABA4B20E610F679154DC97EFD8DE|ytzG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30035 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCCAATCTCTCCTTTA, downstream forward: _UP4_TGACAAAAACCCCGCATACC
• BKK30035 ([gene|69052B24B577ABA4B20E610F679154DC97EFD8DE|ytzG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30035 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCCAATCTCTCCTTTA, downstream forward: _UP4_TGACAAAAACCCCGCATACC

References

Research papers

• 11953756